Application
Western blot analysis of CKAP2 expression in non-transfected: (A) and human CKAP2 transfected: (B) 293T whole cell lysates.| Product Name | CKAP2 Antibody (K-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of CKAP2 of human origin |
| Modification Notes | CKAP2 (cytoskeleton associated protein 2) is localized to the cytoplasm of humans and is expressed in tissues, including thymus and testis. CKAP2 is also refered to as LB1, TMAP or se20-10, and is a 682 amino acid protein which is expressed as three isoforms. CKAP2 is utilized during mitosis and is involved in regulating functions of microtubules, cellular death and the cell cycle. Before mitosis, CKAP2 is expressed in the cell cycle between phases G1 and S, and accumulates between phases S and G2. During mitosis, when the anaphase promoting complex is activated, CKAP2 is degraded. The regulation of CKAP2 is essential for proper spindle functions and cytokinesis, and it is thought that CKAP2 function is mediated via phosphorylation and dephosphorylation. Upon activation of p53 by CKAP2, the G1 phase of the cell cycle is stopped, leading to cell death and apoptosis. Gastric tumors contain excessive amounts of CKAP2, which can lead to unregulated spindle functions may be involved in the development and progression of gastric cancer. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CKAP2 of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of CKAP2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of CKAP2 of human origin |
| Entrez Gene | 26586 |
|---|
Application
Western blot analysis of CKAP2 expression in non-transfected: (A) and human CKAP2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CKAP2 of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
