Application
Western blot analysis of PDE9A expression in non-transfected: (A) and human PDE9A transfected: (B) 293 whole cell lysates and mouse spleen tissue extract (C).Application
Western blot analysis of PDE9A expression in non-transfected: (A) and human PDE9A transfected: (B) 293 whole cell lysates and mouse spleen tissue extract (C).| Product Name | PDE9A Antibody (H-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 424-593 mapping at the C-terminus of PDE9A of human origin |
| Modification Notes | Phosphodiesterases (PDEs) also designated cyclic nucleotide phosphodiesterases, are important for the downregulation of the intracellular level of the second messenger cyclic adenosine monophosphate (cAMP) by hydrolyzing cAMP to 5'AMP. Phosphodiesterase 9A (PDE9A) is a 593 amino acid protein that plays a role in signal transduction via regulation of the intracellular concentration of cyclic nucleotides and has a high affinity for cGMP. There are 15 known isoforms of PDE9A. It is expressed in various tissues including testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate. Highest levels of PDE9A expression occur in brain, kidney, spleen, colon, heart and colon, while there is no detection of PDE9A in blood. PDE9A is composed of an N-terminal regulatory domain and a C-terminal catalytic domain containing two possible divalent metal sites. It may be implicated in affective bipolar disorder. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PDE9A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 424-593 mapping at the C-terminus of PDE9A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 424-593 mapping at the C-terminus of PDE9A of human origin |
| Entrez Gene | 5152 |
|---|
Application
Western blot analysis of PDE9A expression in non-transfected: (A) and human PDE9A transfected: (B) 293 whole cell lysates and mouse spleen tissue extract (C).
Application
Western blot analysis of PDE9A expression in non-transfected: (A) and human PDE9A transfected: (B) 293 whole cell lysates and mouse spleen tissue extract (C).| Application Notes | recommended for detection of PDE9A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
