Application
Western blot analysis of Mds1-Evi-1 expression in CCRF-CEM whole cell lysate.| Product Name | Mds1 Antibody (H-45) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 11-55 mapping near the N-terminus of Mds1 of human origin |
| Modification Notes | The Mds1 and Evi-1 genes located on human chromosome 3q26.2 form a complex locus that encodes three different proteins: Mds1, Evi-1 and a Mds1-Evi-1 fusion protein. Mds1 is a 169 amino acid protein that has lower expression levels than either Mds1-Evi-1 fusion protein or Evi-1. The Mds1-Evi-1 fusion protein is expressed in both normal and leukemic tissues and contains several zinc finger domains. Evi-1 contains two zinc finger domains, the second of which is essential for transactivation of the c-Fos promoter and for AP-1 activation. The first zinc finger domain in Evi-1 binds to Smad3, suppressing its activity and inhibiting TGF∫ signaling. The t(3;21) (q26;q22) chromosomal translocation of Evi-1 produces a chimeric transcription factor, AML-1/Evi-1, that appears to suppress the transactivation of AML-1, which is a stimulator of myeloid cell differentiation. Inappropriate Evi-1 gene expression in hemato-poietic cells has been shown to be associated with acute myelogenous leukemia (AML) and myelodysplastic syndromes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Mds1 and Mds1-Evi-1 fusion protein of mouse and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 11-55 mapping near the N-terminus of Mds1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 11-55 mapping near the N-terminus of Mds1 of human origin |
Application
Western blot analysis of Mds1-Evi-1 expression in CCRF-CEM whole cell lysate.| Application Notes | recommended for detection of Mds1 and Mds1-Evi-1 fusion protein of mouse and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
