Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.Application
Western blot analysis of Integrin β5 expression in non-transfected control (A) and Integrin β5 siRNA transfected (B) HeLa cells. Blot probed with Integrin β5 (H-96): . α-actinin (H-2): used as specificity and loading control.Application
Western blot analysis of Integrin β5 expression in SW480 whole cell lysate.| Product Name | Integrin β5 Antibody (H-96) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 635-730 mapping near the C-terminus of Integrin β5 of human origin |
| Modification Notes | Integrins are heterodimers composed of noncovalently associated transmembrane å and ∫ subunits. The 16 å and 8 ∫ subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including fibronectin, collagen and vitronectin. Certain integrins can also bind to soluble ligands, such as fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Integrin β5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 635-730 mapping near the C-terminus of Integrin &beta 5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 635-730 mapping near the C-terminus of Integrin β5 of human origin |
| Entrez Gene | 3693 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Application
Western blot analysis of Integrin β5 expression in non-transfected control (A) and Integrin β5 siRNA transfected (B) HeLa cells. Blot probed with Integrin β5 (H-96): . α-actinin (H-2): used as specificity and loading control.
Application
Western blot analysis of Integrin β5 expression in SW480 whole cell lysate.| Application Notes | recommended for detection of Integrin β5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
