Application
Western blot analysis of TTBK1 expression in NIH/3T3 (A), BE (2)-M17 (B), SK-N-MC (C), EOC 20 (D), TE671 (E) and C6 (F) whole cell lysates.Application
Western blot analysis of TTBK1 expression in NIH/3T3 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoskeletal localization.| Product Name | TTBK1 Antibody (P-12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of TTBK1 of human origin |
| Modification Notes | TTBK1 (tau tubulin kinase 1), also known as BDTK (brain-derived tau kinase), is a 1,321 amino acid protein that contains one protein kinase domain and belongs to the serine/threonine protein kinase family. Localized to the cytoplasm and expressed at high levels in brain and at lower levels in testis and spinal cord, TTBK1 functions as a serine/threonine kinase that can phosphorylate Tau (a protein involved in tubulin polymerization) on threonine, tyrosine and serine residues. Specifically, TTBK1 uses divalent cations, such as magnesium and manganese, to catalyze the ATP-dependent transfer of a phosphate group onto Tau, creating a phosphoprotein and ADP. Phosphorylation of Tau causes its aggregation and subsequent loss of function, suggesting an important role for TTBK1 in the control of tubulin dynamics. Two isoforms of TTBK1 are expressed due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TTBK1 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of TTBK1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of TTBK1 of human origin |
| Entrez Gene | 84630 |
|---|
Application
Western blot analysis of TTBK1 expression in NIH/3T3 (A), BE (2)-M17 (B), SK-N-MC (C), EOC 20 (D), TE671 (E) and C6 (F) whole cell lysates.
Application
Western blot analysis of TTBK1 expression in NIH/3T3 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoskeletal localization.| Application Notes | recommended for detection of TTBK1 isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
