Application
Western blot analysis of SYP expression in 293T (A) and SH-SY5Y (B) whole cell lysates.Application
Western blot analysis of SYP expression in mouse (A) and rat (B) brain extracts and PC-12 (C) whole cell lysate.Application
Immunofluorescence staining of methanol-fixed PC-12 cells showing membrane staining.| Product Name | SYP Antibody (H-93) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 221-313 mapping at the C-terminus of synaptophysin (major synaptic vesicle protein p38) of human origin |
| Modification Notes | Synaptic vesicles participate in a cycle of fusion with the plasma membrane and reformation by endocytosis. Synaptic vesicle protein synaptophysin (SYP) is targeted to early endosomes in transfected fibroblasts and in neuroendocrine cells. SYP is an N-glycosylated intergral membrane protein found in neurons and endocrine cells that associates into hexamers to form a large conductance channel. SYP contains four transmembrane domains and may function as a gap juction-like channel. Membrane cholesterol specfically interacts with SYP to play a role in vesicle formation. Synaptobrevin (VAMP) also binds to SYP and the resultant complex is upregulated during neuronal development, but is absent in exocytosis fusion complex. Thus, the synaptophysin-synaptobrevin complex is not essential for exocytosis, but rather provides a pool of synaptobrevin for exocytosis. In addition, the tail domain of brain Myosin V also forms a stable complex with synaptobrevin II and SYP, and this complex is disassembled upon the depolarization-induced entry of Ca2+ into intact nerve endings. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of synaptophysin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 221-313 mapping at the C-terminus of synaptophysin (major synaptic vesicle protein p38) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 221-313 mapping at the C-terminus of synaptophysin (major synaptic vesicle protein p38) of human origin |
| Entrez Gene | 6855 |
|---|
Application
Western blot analysis of SYP expression in 293T (A) and SH-SY5Y (B) whole cell lysates.
Application
Western blot analysis of SYP expression in mouse (A) and rat (B) brain extracts and PC-12 (C) whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed PC-12 cells showing membrane staining.| Application Notes | recommended for detection of synaptophysin of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
