Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubules.Application
Western blot analysis of MYH9 expression in rat skeletal muscle tissue extract.| Product Name | MYH9 Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1741-1780 mapping near the C-terminus of myosin heavy chain 9 of human origin |
| Modification Notes | Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of 2 heavy chains (MHC) and 4 light chains (MLC) that interacts with actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Myosin heavy chain 9 can also be designated Myosin IIa, nonmuscle myosin heavy chain IIa, Cellular myosin heavy chain, type A, myosin-9 or NMMHC-IIA. Myosin heavy chain 9 is involved in cell shape, cytokinesis, and specialized functions such as capping and secretion. It is expressed in leukoctyes and in kidney glomeruli. Defects in the MYH9 gene, which encodes myosin heavy chain 9, are linked to Sebastian syndrome (SBS), Fechtner syndrome (FTNS), Alport syndrome with macrothrombocytopenia (APSM), autosomal dominant nonsyndromic sensorineural deafness 17 (DFNA17) and Epstein syndrome (EPS). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of myosin heavy chain 9 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1741-1780 mapping near the C-terminus of myosin heavy chain 9 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1741-1780 mapping near the C-terminus of myosin heavy chain 9 of human origin |
| Entrez Gene | 4627 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubules.
Application
Western blot analysis of MYH9 expression in rat skeletal muscle tissue extract.| Application Notes | recommended for detection of myosin heavy chain 9 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
