Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) programApplication
Western blot analysis of Xanthine Oxidase expression in rat liver tissue extract.| Product Name | Xanthine Oxidase Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 251-360 mapping near the N-terminus of Xanthine Oxidase of human origin |
| Modification Notes | The process of metabolizing purines to a common molecule known as xanthine is an essential process for the proper shuttling of uric acid (1,2). Xanthine oxidase is a flavoprotein enzyme that coordinates molybdenum and utilizes NAD+ as an electron acceptor to catalyze the oxidation of hypoxanthine to xanthine and then to uric acid (1,2). The predominant form of this enzyme is xanthine dehydrogenase, which is a homodimer that can be converted to xanthine oxidase by sulfhydryl oxidation or proteolytic modification (1,2). Xanthine oxidase is present in species ranging from bacteria to human and is ubiquitously expressed in mammalian tissues (3,4). In the oxidase form, this enzyme is coupled to the generation of free radicals (5). Individuals showing marked elevation of serum xanthine oxidase is suggestive of chronic liver disease and cholestasis, which is a condition defined by hepatic obstruction (6,7). Hepatic obstruction causes bile salts, the bile pigment bilirubin, and fats to accumulate in the blood stream instead of being eliminated normally (6,7). The clinical consequences of defects in xanthine oxidase range from mild to severe and even contribute to fatal disorders (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Xanthine Oxidase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 251-360 mapping near the N-terminus of Xanthine Oxidase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 251-360 mapping near the N-terminus of Xanthine Oxidase of human origin |
| Entrez Gene | 7498 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program
Application
Western blot analysis of Xanthine Oxidase expression in rat liver tissue extract.| Application Notes | recommended for detection of Xanthine Oxidase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
