Application
Western blot analysis of HPRT expression in non-transfected: (A) and human HPRT transfected: (B) 293 whole cell lysates.Application
Western blot analysis of HPRT expression in non-transfected 293T: (A), mouse HPRT transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of HPRT expression in non-transfected 293T: (A), mouse HPRT transfected 293T: (B) and A549 (C) whole cell lysates.Application
Western blot analysis of HPRT expression in MIA PaCa-2 (A) and A549 (B) whole cell lysates.| Product Name | HPRT Antibody (FL-218) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-218 representing full length HPRT of human origin |
| Modification Notes | HPRT (hypoxanthine phosphoribosyltransferase 1), also known as HGPRT or HPRT1, is a 218 amino acid cytoplasmic protein that belongs to the purine/pyrimidine phosphoribosyltransferase family. Involved in purine metabolism, HPRT functions as a purine salvage enzyme that catalyzes the conversion of hypoxathine and guanine to their respective mononucleotides (inosine monophosphate and guanosine monophosphate, respectively). HPRT exists as a homotetramer that can bind two magnesium ions as cofactors. Defects in the gene encoding HPRT are the cause of gout and Lesch-Nyhan syndrome (LNS), both of which are characterized by a partial or complete lack of NPRT enzymatic activity. While a partial loss of HPRT enzymatic activity results in a buildup of uric acid (gout), a total loss of enzymatic activity results in hyperuricaemia, mental retardation, choreoathetosis and compulsive self-mutilation, all of which are symptoms associated with LNS. The severity of these diseases suggests an essential role for HPRT in purine metabolism. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HPRT of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-218 representing full length HPRT of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-218 representing full length HPRT of human origin |
| Entrez Gene | 3251 |
|---|
Application
Western blot analysis of HPRT expression in non-transfected: (A) and human HPRT transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of HPRT expression in non-transfected 293T: (A), mouse HPRT transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of HPRT expression in non-transfected 293T: (A), mouse HPRT transfected 293T: (B) and A549 (C) whole cell lysates.
Application
Western blot analysis of HPRT expression in MIA PaCa-2 (A) and A549 (B) whole cell lysates.| Application Notes | recommended for detection of HPRT of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
