Application
Western blot analysis of VAC14 expression in non-transfected: (A) and mouse VAC14 transfected: (B) 293T whole cell lysates.| Product Name | VAC14 Antibody (H-261) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 421-681 mapping within an internal region of VAC14 of human origin |
| Modification Notes | Phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) is a signaling molecule that exists as a minor component of cell membranes and is essential for the distinguishing of cellular compartments. The synthesis of PI(3,5)P2 is regulated by a number of proteins that are involved in intracellular trafficking and assembly events throughout the cell. VAC14, also known as TAX1BP2 (Tax1-binding protein 2) or TRX, is a 782 amino acid protein that contains six HEAT repeats and exists as part of a regulatory complex with FIG4. Expressed ubiquitously, VAC14 works with FIG4 to control the synthesis of PI(3,5)P2, specifically mediating the activation of PIP5KIII, a kinase involved in the regulation of PI(3,5)P2 activity. The gene encoding VAC14 maps to human chromosome 16, which houses over 900 genes and comprises nearly 3% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of VAC14 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 421-681 mapping within an internal region of VAC14 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 421-681 mapping within an internal region of VAC14 of human origin |
| Entrez Gene | 55697 |
|---|
Application
Western blot analysis of VAC14 expression in non-transfected: (A) and mouse VAC14 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of VAC14 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
