Application
Western blot analysis of MADD expression in HEK293 whole cell lysate.Application
Western blot analysis of MADD expression in non-transfected: (A) and mouse MADD transfected: (B) 293T whole cell lysates.| Product Name | MADD Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-298 mapping at the N-terminus of MADD of human origin |
| Modification Notes | MADD (MAP-kinase activating death domain), also known as DENN, IG20 or KIAA0358, is a 1,647 amino acid multi-pass membrane protein that contains one DENN domain and one death domain and belongs to the MADD family. Expressed at high levels in adult testis, heart and ovary, as well as in fetal brain and kidney, MADD interacts with TNF-R1 and plays an important role in cell proliferation, survival and death, specifically by regulating alternative splicing events. Overexpression of MADD stimulates the mitogen-activated protein (MAP) kinase extracellular signal-regulated kinase (ERK), thereby influencing MAP kinase signaling cascades. Multiple isoforms of MADD exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MADD of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-298 mapping at the N-terminus of MADD of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-298 mapping at the N-terminus of MADD of human origin |
| Entrez Gene | 8567 |
|---|
Application
Western blot analysis of MADD expression in HEK293 whole cell lysate.
Application
Western blot analysis of MADD expression in non-transfected: (A) and mouse MADD transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of MADD of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
