Application
Western blot analysis of AHCYL1 expression in non-transfected: (A) and human AHCYL1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AHCYL1 expression in non-transfected: (A) and human AHCYL1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AHCYL1 expression in non-transfected: (A) and mouse AHCYL1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AHCYL1/2 expression in HeLa (A) and ME-180 (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Product Name | AHCYL1/2 Antibody (H-160) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-160 mapping at the N-terminus of AHCYL1 of human origin |
| Modification Notes | AHCYL1 (S-adenosylhomocysteine hydrolase-like 1), also known as DCAL, IRBIT or PRO0233, and AHCYL2 (S-adenosylhomocysteine hydrolase-like 2) are endoplasmic reticulum (ER) proteins involved in amino acid biosynthesis. Expressed in dentritic blood cells (DCs), AHCYL1 and AHCYL2 function to catalyze the H(2)O-dependent conversion of S-adenosyl-L-homocysteine to L-homocysteine and adenosine, a reaction that uses NAD as a cofactor. Additionally, AHCYL1 contains a PDZ-binding domain and a PEST region through which it can interact with IP3R-I (inositol 1,4,5-trisphosphate (IP3) receptor-I), a protein involved in various signaling pathways. This interaction lowers the affinity of IP3R-1 for its substrate, IP3, thereby decreasing the rate of IP3-IP3R-I binding. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AHCYL1 and AHCYL2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-160 mapping at the N-terminus of AHCYL1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-160 mapping at the N-terminus of AHCYL1 of human origin |
Application
Western blot analysis of AHCYL1 expression in non-transfected: (A) and human AHCYL1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AHCYL1 expression in non-transfected: (A) and human AHCYL1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AHCYL1 expression in non-transfected: (A) and mouse AHCYL1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AHCYL1/2 expression in HeLa (A) and ME-180 (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.| Application Notes | recommended for detection of AHCYL1 and AHCYL2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
