Application
Western blot analysis of MDHC expression in HS 181.Tes (A), NTERA-2 cl.D1 (B), F9 (C) and HL-60 (D) whole cell lysates and mouse brain tissue extract (E).Application
Western blot analysis of MDHC expression in mouse testis tissue extract (A) and Jurkat whole cell lysate (B).| Product Name | MDHC Antibody (H-54) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 267-320 mapping near the C-terminus of MDHC of human origin |
| Modification Notes | Cytosolic malate dehydrogenase (MDHC or cMDH) is an important NAD-dependent enzyme involved in glycometabolism that catalyzes the formation of oxaloacetate and NADH from L-malate and NAD. MDHC is highly expressed in brain, heart and skeletal muscle and plays a role in aerobic energy production for muscle contraction, transmission of neuronal signals, absorption/ resorption pathways, collagen-supporting functions, dead cell phagocytosis, as well as pathways involved in gas exchange and cell division. Furthermore, MDHC is a regulatory subunit of the nucleic acid-conducting channel (NACh). MDHC functions as a homodimer and is highly conserved in plants, animals and bacteria. The activity of MDHC is controlled by the sesquiterpenoid juvenile hormone (JH) and the steroid hormone ecdysone. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MDHC (malate dehydrogenase, cytoplasmic) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 267-320 mapping near the C-terminus of MDHC of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 267-320 mapping near the C-terminus of MDHC of human origin |
| Entrez Gene | 4190 |
|---|
Application
Western blot analysis of MDHC expression in HS 181.Tes (A), NTERA-2 cl.D1 (B), F9 (C) and HL-60 (D) whole cell lysates and mouse brain tissue extract (E).
Application
Western blot analysis of MDHC expression in mouse testis tissue extract (A) and Jurkat whole cell lysate (B).| Application Notes | recommended for detection of MDHC (malate dehydrogenase, cytoplasmic) of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
