| Product Name | GGTase-IIβ Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 139-331 mapping at the C-terminus of GGTase-IIβ of human origin |
| Modification Notes | Eukaryotic cells contain three distinct prenyltransferases that catalyze the attachment of a thioether-linked 15-carbon farnesyl group or 20-carbon geranylgeranyl group to C-terminal cysteine residues. Geranylgeranyltransferase type I (GGTase-I, PGGTase-I) catalyzes the nucleophilic substitution reaction between geranylgeranyl diphosphate (GGPP) and a protein-derived thiol to form the thioether linkage. The candidate protein contains a C-terminal CAAX motif in which “A” is an aliphatic amino acid and “X” is leucine. Geranylgeranylation is necessary for the TGF∫1 signaling pathway, which involves phosphatidylcholine-specific phospholipase and a protein kinase C. Human GGTase-I contains an å subunit and a ∫ subunit. Geranylgeranyltransferase type II (GGTase-II) is a heterodimer that catalyzes the transfer of two 20-carbon geranylgeranyl groups from geranylgeranyl pyrophosphate onto C-terminal cysteine residues of Rab GTPases, which is required for the activity of Rab proteins. GGTase-II also contains an å subunit and a ∫ subunit. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GGTase-IIβ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 139-331 mapping at the C-terminus of GGTase-II&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 139-331 mapping at the C-terminus of GGTase-IIβ of human origin |
| Application Notes | recommended for detection of GGTase-IIβ of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
