Application
Western blot analysis of Phocein expression in NIH/3T3 whole cell lysate.| Product Name | Phocein Antibody (FL-225) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-225 representing full length Phocein of human origin |
| Modification Notes | Phocein (preimplantation protein 3, Mps1 binder kinase activator-like 3, 2C4D) is a 225 amino acid protein encoded by the human gene MOBKL3. Phocein belongs to the MOB1/phocein family and is phosphorylated on serine residues. Phocein is a widely expressed, highly conserved intracellular protein. The sequence of Phocein has limited homology to the sigma subunits from Clathrin adaptor complexes and contains an additional stretch bearing a putative SH3-binding domain. Phocein is usually associated with membranes but can be present in the cytosol, where it behaves as a protein complex. Phocein is the major partner of the striatin family members, which are scaffolding proteins involved in signaling and trafficking. Due to its association with Dynamin via direct interactions with nucleotide diphosphate kinase (NDPK) and Eps15, Phocein has been implicated in vesicular trafficking, acting in particular in the endocytic process. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Phocein of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-225 representing full length Phocein of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-225 representing full length Phocein of human origin |
| Entrez Gene | 25843 |
|---|
Application
Western blot analysis of Phocein expression in NIH/3T3 whole cell lysate.| Application Notes | recommended for detection of Phocein of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
