Application
Western blot analysis of TRPM8 expression in non-transfected (A) and human TRPM8 transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma tissue showing cytoplasmic and membrane localization.| Product Name | TRPM8 Antibody (D-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of TRPM8 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Transient receptor potential ion channels (TRPC) are a superfamily of six transmembrane segment-spanning gated cation channels. TRP subtypes mediate store-operated Ca2+ entry, a process involving Ca2+ influx and replenishment of Ca2+ stores formerly emptied through the action of inositol 1,4,5-trisphosphate production and other Ca2+ mobilizing agents. TRP ion channels influence calcium-depletion-induced calcium influx processes in response to chemo-, mechano- and osmo-regulatory events. TRPM8 (transient receptor potential cation channel, subfamily M, member 8), also known as TRPP8 or LTRPC6, is a 1,104 amino acid multi-pass membrane protein that belongs to the TRP family of regulatory channel proteins. Expressed at high levels in prostate, TRPM8 functions as a receptor-activated cation channel that is permeable to monovalent cations (such as sodium and potassium) and divalent Ca2+ and is involved in the detection of temperature sensations (such as the feeling of coolness) throughout the body. TRPM8 is overexpressed in prostate tumors, as well as in colon, breast and lung cancers, suggesting an important role for TRPM8 in tumorigenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TRPM8 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of TRPM8 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 79054 |
|---|
Application
Western blot analysis of TRPM8 expression in non-transfected (A) and human TRPM8 transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human prostate carcinoma tissue showing cytoplasmic and membrane localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
