Application
Western blot analysis of ACOX3 expression in non-transfected: (A) and mouse ACOX3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ACOX3 expression in non-transfected 293T: (A), mouse ACOX3 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of ACOX3 expression in non-transfected 293T: (A), human ACOX3 transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | ACOX3 Antibody (M-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 431-630 mapping near the C-terminus of ACOX3 of mouse origin |
| Modification Notes | ACOX3 (acyl-Coenzyme A oxidase 3), also known as BRCOX or PRCOX, is a 700 amino acid protein that localizes to peroxisomes and belongs to the acyl-CoA oxidase family. Using FAD as a cofactor, ACOX3 catalyzes the desaturation of 2-methyl branched fatty acids in peroxisomes, thereby playing an important role in peroxisomal fatty acid beta-oxidation. Human ACOX3 shares 75% sequence identity with its rat counterpart, suggesting a conserved role between species. Multiple isoforms of ACOX3 exist due to alternative splicing events. The gene encoding ACOX3 maps to human chromosome 4, which encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ACOX3 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 431-630 mapping near the C-terminus of ACOX3 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 431-630 mapping near the C-terminus of ACOX3 of mouse origin |
Application
Western blot analysis of ACOX3 expression in non-transfected: (A) and mouse ACOX3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ACOX3 expression in non-transfected 293T: (A), mouse ACOX3 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of ACOX3 expression in non-transfected 293T: (A), human ACOX3 transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of ACOX3 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
