| Product Name | Topo IIIα Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 702-1001 of Topo IIIα of human origin |
| Modification Notes | DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA by transiently breaking and rejoining DNA strands. Although DNA topoisomerase I (Topo I) and Topo II relax both positive and negative supercoils, Topo III relaxes only negative supercoils. Topo IIIå exists as a long and a short isoform, which are produced by alternative splicing. Topo IIIå, which localizes to the nucleolus, is constitutively expressed and remains at high levels throughout the cell cycle in HL-60 cells. Topo III∫ exists as three isoforms, ∫-1, ∫-2 and ∫-3, also produced by alternative splicing. Topo III∫-1 is expressed in testis, heart, and skeletal muscle, whereas Topo III∫-2 is expressed in thymus, kidney and pancreas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DNA Topoisomerase IIIα of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 702-1001 of Topo III&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 702-1001 of Topo IIIα of human origin |
| Entrez Gene | 7156 |
|---|
| Application Notes | recommended for detection of DNA Topoisomerase IIIα of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
