Application
Western blot analysis of CMAH expression in mouse kidney tissue extract.| Product Name | CMAH Antibody (M-240) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 3-242 mapping near the N-terminus of CMAH of mouse origin |
| Modification Notes | The sialic acids are a family of acidic sugars typically found in the outer portion of the cell surface and in secreted glycoconjugates of all vertebrates. Cell membrane sialic acid is involved in cell-cell and cell-pathogen interactions and in binding of cells to the extracellular matrix. The two most common forms of sialic acid found in mammalian cells are N-acetylneuraminic acid (Neu5Ac) and its hydroxylated derivative, N-glycolylneuraminic acid (Neu5Gc). CMAH (cytidine monophospho-N-acetylneuraminic acid hydroxylase), also known as CMP-Neu5Ac hydroxylase or CMP-N-acetylneuraminate monooxygenase, is a 577 amino acid cytoplasmic protein that is expressed in all tissues, except in brain. Belonging to the CMP-Neu5Ac hydroxylase family, CMAH catalyzes the conversion of CMP-Neu5Ac into its hydroxylated derivative CMP-Neu5Gc, a sialic acid abundantly expressed at the surface of many cells. CMAH exists as two isoforms due to alternative splicing events. Isoform 2 is expressed in the endoplasmic reticulum. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CMAH of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 3-242 mapping near the N-terminus of CMAH of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 3-242 mapping near the N-terminus of CMAH of mouse origin |
Application
Western blot analysis of CMAH expression in mouse kidney tissue extract.| Application Notes | recommended for detection of CMAH of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
