Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of HDAC6 expression in 293 whole cell lysate (A) and K-562 nuclear extract (B).Application
Western blot analysis of HDAC6 expression in non-transfected 293T: (A), human HDAC6 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of HDAC6 expression in K-562 whole cell lysate.| Product Name | HDAC6 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 916-1215 mapping at the C-terminus of HDAC6 of human origin |
| Modification Notes | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBPassociated factor), p300/CBP, HAT1, and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6, have been identified as histone deacetylases. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of HDAC6 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 916-1215 mapping at the C-terminus of HDAC6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 916-1215 mapping at the C-terminus of HDAC6 of human origin |
| Entrez Gene | 10013 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of HDAC6 expression in 293 whole cell lysate (A) and K-562 nuclear extract (B).
Application
Western blot analysis of HDAC6 expression in non-transfected 293T: (A), human HDAC6 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of HDAC6 expression in K-562 whole cell lysate.| Application Notes | recommended for detection of HDAC6 of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
