Application
Western blot analysis of STAMBP expression in non-transfected 293T: (A), human STAMBP transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of STAMBP expression in non-transfected 293T: (A), mouse STAMBP transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of STAMBP expression in non-transfected 293T: (A), mouse STAMBP transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of STAMBP expression in non-transfected: (A) and human STAMBP transfected: (B) 293T whole cell lysates.Application
Western blot analysis of STAMBP expression in HeLa whole cell lysate.| Product Name | STAMBP Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 161-270 mapping within an internal region of STAMBP of human origin |
| Modification Notes | STMBP (STAM binding protein), also known as AMSH, is a 424 amino acid protein belonging to the peptidase M67C family. Ubiquitously expressed, STMBP functions as a zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. STMBP is able to oppose the ubiquitin-dependent sorting of receptors to lysosomes. STMBP may play a role in signal transduction for cell growth and Myc induction mediated by IL-2 and GM-CSF. It is suggested that STMBP potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of Smad6 and Smad7. STMBP consists of the JAMM motif, which is essential for the protease activity, and is inhibited by N-ethylmaleimide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of STAMBP of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 161-270 mapping within an internal region of STAMBP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 161-270 mapping within an internal region of STAMBP of human origin |
| Entrez Gene | 10617 |
|---|
Application
Western blot analysis of STAMBP expression in non-transfected 293T: (A), human STAMBP transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of STAMBP expression in non-transfected 293T: (A), mouse STAMBP transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of STAMBP expression in non-transfected 293T: (A), mouse STAMBP transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of STAMBP expression in non-transfected: (A) and human STAMBP transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of STAMBP expression in HeLa whole cell lysate.| Application Notes | recommended for detection of STAMBP of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
