Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of cells in mole-cular layer and Purkinje cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of EF-1 α2 expression in non-transfected 293T: (A), human EF-1 α2 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of EF-1 α2 expression in HEK293 whole cell lysate (A) and SK-N-MC (B), IMR-32 (C) and Sol8 (D) nuclear extracts and rat skeletal muscle (E) and mouse heart (F) tissue extracts.| Product Name | EF-1 α2 Antibody (D-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of EF-1 α2 of human origin |
| Modification Notes | Elongation factor 1 alpha 2 (EF-1 å2) is a eukaryotic protein translation factor that is expressed in terminally differentiated cells of skeletal muscle, heart, and certain areas of the brain. Along with protein synthesis, EF-1 å2 also plays a role in cytoskeletal remodelling and apoptosis. The gene encoding for EF-1 å2 maps to chromosome 20q13. Wasted (wst) refers to a spontaneous autosomal recessive mutation in which this gene is deleted, and it leads to tremors and disturbances of gait shortly after weaning, followed by motor neuron degeneration, paralysis, and death by about 28 days in mice. EF-1 å2 has the ability to transform mammalian cells and is highly expressed in tumors of the ovary, breast, and lung, thereby proving a good candidate for an oncogene. In addition to this, EF-1 å2 enhances focus formation, allows anchorage-independent growth, and decreases the doubling time of rodent fibroblasts. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of EF-1 α2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of EF-1 &alpha 2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of EF-1 α2 of human origin |
| Entrez Gene | 1917 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of cells in mole-cular layer and Purkinje cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of EF-1 α2 expression in non-transfected 293T: (A), human EF-1 α2 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of EF-1 α2 expression in HEK293 whole cell lysate (A) and SK-N-MC (B), IMR-32 (C) and Sol8 (D) nuclear extracts and rat skeletal muscle (E) and mouse heart (F) tissue extracts.| Application Notes | recommended for detection of EF-1 α2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
