Application
Western blot analysis of Ep-CAM expression in non-transfected: (A) and human Ep-CAM transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Ep-CAM expression in non-transfected 293T: (A), human Ep-CAM transfected 293T: (B) and A-431 (C) whole cell lysates.| Product Name | Ep-CAM Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 24-93 mapping near the N-terminus of Ep-CAM of human origin |
| Modification Notes | The epithelial cell adhesion molecule Ep-CAM, which is also designated tumor-associated calcium signal transducer 1 and MK-1, is a monomeric membrane glycoprotein that is expressed in most normal human epithelium and carcinomas. The human Ep-CAM gene encodes a 314 amino acid protein that is expressed as two forms, a major form and a minor form, which are reduced upon treatment with the amino-glycosylation inhibitor tunicamycin. Ep-CAM is overexpressed in a variety of carcinomas and is, therefore, a potential target for the visualization and therapy of human solid tumors. Ep-CAM contains an extracellular domain containing two epidermal growth factor-like repeats, followed by a cysteine poor region, which is necessary for the adhesion properties of the molecule. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ep-CAM of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 24-93 mapping near the N-terminus of Ep-CAM of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 24-93 mapping near the N-terminus of Ep-CAM of human origin |
| Entrez Gene | 4072 |
|---|
Application
Western blot analysis of Ep-CAM expression in non-transfected: (A) and human Ep-CAM transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Ep-CAM expression in non-transfected 293T: (A), human Ep-CAM transfected 293T: (B) and A-431 (C) whole cell lysates.| Application Notes | recommended for detection of Ep-CAM of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
