Application
Western blot analysis of Jun B phosphorylation in non-transfected: (A) and human Jun B transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Jun B phosphorylation in non-transfected: (A) and human Jun B transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Jun B phosphorylation in non-transfected: (A) and mouse Jun B transfected: (B) 293T whole cell lysates.Application
Blots were probed with p-Jun B (Ser 259): (A) p-Jun B (Ser 259): preincubated with its cognate non-phosphorylated peptide (B) and p-Jun B (Ser 259): preincubated with its cognate phosphorylated peptide (C).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.| Product Name | p-Jun B Antibody (Ser 259) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 259 of Jun B of human origin |
| Modification | Phosphoralated |
| Modification Notes | The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein along with c-Fos is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 259 phosphorylated Jun B of human origin and correspondingly phosphorylated Ser 256 of mouse and rat origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 259 of Jun B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 259 of Jun B of human origin |
| Entrez Gene | 3726 |
|---|
Application
Western blot analysis of Jun B phosphorylation in non-transfected: (A) and human Jun B transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Jun B phosphorylation in non-transfected: (A) and human Jun B transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Jun B phosphorylation in non-transfected: (A) and mouse Jun B transfected: (B) 293T whole cell lysates.
Application
Blots were probed with p-Jun B (Ser 259): (A) p-Jun B (Ser 259): preincubated with its cognate non-phosphorylated peptide (B) and p-Jun B (Ser 259): preincubated with its cognate phosphorylated peptide (C).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.| Application Notes | recommended for detection of Ser 259 phosphorylated Jun B of human origin and correspondingly phosphorylated Ser 256 of mouse and rat origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
