Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear staining of lymphoid and epithelial cells at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear staining of lymphoid and epithelial cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of IRF-2 expression in non-transfected: (A) and mouse IRF-2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of IRF-2 expression in non-transfected: (A) and human IRF-2 transfected: (B) 293T whole cell lysates.| Product Name | IRF-2 Antibody (H-229) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 121-349 of IRF-2 of human origin |
| Modification Notes | Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-å and ∫) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-å and interferon-∫ genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3© p48 and IFN consensus sequence-binding protein (ICSBP). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of IRF-2 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 121-349 of IRF-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 121-349 of IRF-2 of human origin |
| Entrez Gene | 3660 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear staining of lymphoid and epithelial cells at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing nuclear staining of lymphoid and epithelial cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of IRF-2 expression in non-transfected: (A) and mouse IRF-2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of IRF-2 expression in non-transfected: (A) and human IRF-2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of IRF-2 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
