Application
Western blot analysis of β-Amyloid expression in H4 (A), PC-3 (B) and U-87 MG (C) whole cell lysates.Application
Western blot analysis of β-Amyloid expression in H4 whole cell lysate (A) and human recombinant β-Amyloid fusion protein (B).| Product Name | β-Amyloid Antibody (H-43) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 672-714 of Amyloid A4 representing full length β-Amyloid protein of human origin |
| Modification Notes | Proteolytic cleavage of the Amyloid protein precursor (APP) gives rise to the ∫-Amyloid and Amyloid A4 proteins, which are present in human platelets. Amyloid deposition is associated with type II diabetes, Down syndrome and a variety of neurological disorders, including Alzheimer’s disease. The Amyloid precursor protein (APP) undergoes alternative splicing, resulting in several isoforms. Proteolytic cleavage of APP leads to the formation of the Amyloid ∫/A4 Amyloid protein. This protein is involved in the formation of neurofibrillary tangles and plaques that characterize the senile plaques of Alzheimer’s patients. APLP1 (Amyloid precursor-like protein 1) and APLP2 are structurally similar to APP. Human APLP2 is a membrane- bound sperm protein that contains a region highly homologous to the transmembrane-cytoplasmic domains of APP found in brain plaques of Alzheimer’s disease patients. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of 4 kDa β-Amyloid and Amyloid A4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 672-714 of Amyloid A4 representing full length &beta -Amyloid protein of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 672-714 of Amyloid A4 representing full length β-Amyloid protein of human origin |
| Entrez Gene | 351 |
|---|
Application
Western blot analysis of β-Amyloid expression in H4 (A), PC-3 (B) and U-87 MG (C) whole cell lysates.
Application
Western blot analysis of β-Amyloid expression in H4 whole cell lysate (A) and human recombinant β-Amyloid fusion protein (B).| Application Notes | recommended for detection of 4 kDa β-Amyloid and Amyloid A4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
