Application
Western blot analysis of cyclin E2 expression in Jurkat (A), IMR-32 (B) and HeLa (C) nuclear extracts.| Product Name | cyclin E2 Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-140 of cyclin E2 of human origin |
| Modification Notes | Cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. Cyclin E2 is a cyclin E-related protein that specifically interacts with Cdk2 and Cdk3 and with p27 and p21. Cyclin E2 expression peaks at the G1/S phase transition of the cell cycle, in parallel with cyclin E. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of cyclin E2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-140 of cyclin E2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-140 of cyclin E2 of human origin |
| Entrez Gene | 9134 |
|---|
Application
Western blot analysis of cyclin E2 expression in Jurkat (A), IMR-32 (B) and HeLa (C) nuclear extracts.| Application Notes | recommended for detection of cyclin E2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
