Application
Western blot analysis of cyclin G1 expression in Jurkat nuclear extract.Application
Western blot analysis of cyclin G1 expression in HeLa nuclear extract (A) and HeLa (B) and ES-2 (C) whole cell lysates.| Product Name | cyclin G1 Antibody (C-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of cyclin G1 of rat origin |
| Modification Notes | Cyclins are the regulatory subunits of Cdc2 p34 and related cyclin-dependent kinases (Cdks) which play critical roles in the control of cell cycle progression. The catalytic subunit for cyclin A and B is Cdc2 p34 kinase. The Cdc2-cyclin B complex controls the G2 to M transition whereas Cdc2-cyclin A regulates S phase progression. The G1 to S transition, however, appears to be controlled by the G1 cyclins. cyclin D1 accumulates during G1 and associates with Cdk2, Cdk4 and Cdk5. cyclin E and Cdk2 interact during the G1 to S transition. cyclin G contains a typical N terminal cyclin box and a carboxy-terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor. cyclin G expression is induced within 3 hours after growth stimulation and remains elevated with no apparent cell cycle dependency. cyclin G2 shares 53% amino acid sequence identity with cyclin G1. Peak expression of cyclin G2 is seen in late S phase, as opposed to cyclin G1 expression, which is constitutive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of cyclin G1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of cyclin G1 of rat origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of cyclin G1 of rat origin |
| Entrez Gene | 900 |
|---|
Application
Western blot analysis of cyclin G1 expression in Jurkat nuclear extract.
Application
Western blot analysis of cyclin G1 expression in HeLa nuclear extract (A) and HeLa (B) and ES-2 (C) whole cell lysates.| Application Notes | recommended for detection of cyclin G1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
