Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): , p300 (C-20): 585, p300 (N-15): 584, p300 (H-272): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , Bcl-3 (C-14): , Bcl-3 (H-146): , PCAF (C-16): , PCAF (H-369): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.Application
Antibodies tested include β-catenin (H-102): , β-catenin (C-18): , β-catenin (E-5): , Tip60 (N-17): , TRRAP (T-17): , TRRAP (Y-18): , TRRAP (F-20): , TRRAP (H-300): , CBP (A-22): , CBP (C-20): , CBP (451): , CPB (C-1): , p300 (H-272): , p300 (N-15): and p300 (C-20): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.Application
Western blot analysis of truncated rat recombinant CBP CREB binding domain fusion protein.| Product Name | CBP Antibody (451) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | raised against amino acids 451-720 mapping within the CREB Binding Domain of CBP of mouse origin. |
| Modification Notes | Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression, even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of CBP p265 and p300 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against amino acids 451-720 mapping within the CREB Binding Domain of CBP of mouse origin. |
|---|---|
| Tissue Specificity | raised against amino acids 451-720 mapping within the CREB Binding Domain of CBP of mouse origin. |
| Entrez Gene | 1387 |
|---|
Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): , p300 (C-20): 585, p300 (N-15): 584, p300 (H-272): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Application
Antibodies tested include NFκB p50 (C-19): , NFκB p50 (E-10): , NFκB p50 (H-119): , NFκB p65 (C-20): , NFκB p65 (A): , NFκB p65 (H-286): , Bcl-3 (C-14): , Bcl-3 (H-146): , PCAF (C-16): , PCAF (H-369): , CBP (A-22): , CBP (C-1): , CBP (C-20): , CBP (451): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Baek et al., Cell 2002, 110: 55-67.
Application
Antibodies tested include β-catenin (H-102): , β-catenin (C-18): , β-catenin (E-5): , Tip60 (N-17): , TRRAP (T-17): , TRRAP (Y-18): , TRRAP (F-20): , TRRAP (H-300): , CBP (A-22): , CBP (C-20): , CBP (451): , CPB (C-1): , p300 (H-272): , p300 (N-15): and p300 (C-20): . Data kindly provided by M.G. Rosenfeld and reproduced with permission from Kioussi et al., Cell 2002, 111: 673-685.
Application
Western blot analysis of truncated rat recombinant CBP CREB binding domain fusion protein.| Application Notes | recommended for detection of CBP p265 and p300 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
