Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of ADHγ expression in non-transfected: (A) and human ADHγ transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.Application
Western blot analysis of ADH expression in Hep G2 whole cell lysate.| Product Name | ADH Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 of ADHα of human origin |
| Modification Notes | The alcohol dehydrogenase family of proteins metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products (1). Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism (1). Three genes encoding alpha (ADH1A), beta (ADH1B) and gamma (ADH1C) subunits are tandemly organized on chromosome 4q22 as a gene cluster (1–4). The alpha form of ADH is monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood (5). The genes encoding beta and gamma subunits, however, are polymorphic and strongly expressed in adult livers (5,6). With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohols to ketones or aldehydes (5). The physiologic function for ADH in the liver is the removal of ethanol formed by microorganisms in the intestinal tract (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of ADHα, ADHβ and ADHγ of human origin and ADH1 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA. also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of ADH&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of ADHα of human origin |
| Entrez Gene | 124 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of ADHγ expression in non-transfected: (A) and human ADHγ transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.
Application
Western blot analysis of ADH expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of ADHα, ADHβ and ADHγ of human origin and ADH1 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA. also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
