Application
Western blot analysis of p-NDEL1 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | p-NDEL1 Antibody (Ser 242) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 242 phosphorylated NDEL1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification | Phosphoralated |
| Modification Notes | NudE-like protein (NDEL1) is expressed in testis, brain, heart, hypothalamus, liver, lung, spleen, and stomach, specifically in the interphase centrosome and mitotic spindle. It positively regulates minus-end directed dynein. Evidence suggests that NDEL1 interacts with LIS1 to sustain the function of dynein, thereby impacting microtubule organization, nuclear translocation, and neuronal positioning. NDEL1 is phosphorylated during mitosis and seems to tether dynactin and dynein to the mother centriole for microtubule anchoring. Loss of function of NDEL1 in the developing neocortex impairs neuronal positioning and uncouples the centrosome and nucleus. NDEL1 may also impair mitochondrial transport or function, initiating a cascade of events culminating in psychiatric illness such as lissencephaly and schizophrenia. Human NDEL1 is subject to phosphorylation at a variety of amino acid residues, including Ser 242. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 242 phosphorylated NDEL1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Ser 242 phosphorylated NDEL1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 81565 |
|---|
Application
Western blot analysis of p-NDEL1 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
