Application
Western blot analysis of AlaRS expression in non-transfected 293T: (A), human AlaRS transfected 293T: (B), MCF7 (C), HeLa (D), K-562 (E) and Hep G2 (F) whole cell lysates.Application
Western blot analysis of AlaRS expression in HeLa whole cell lysate.Application
Western blot analysis of AlaRS expression in Caki-1 (A), K-562 (B) and Hep G2 (C) whole cell lysates.| Product Name | AlaRS Antibody (H-268) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 701-968 mapping at the C-terminus of AlaRS of human origin |
| Modification Notes | Aminoacyl-tRNA synthetases function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. Class II tRNA synthases are a highly conserved subfamily of tRNA synthetases that have a catalytic domain through which they interact with the amino acid acceptor of the tRNA and a second domain through which they interact with the rest of the tRNA molecule. AlaRS (alanyl-tRNA synthetase), also known as AARS, is a 968 amino acid cytoplasmic protein that belongs to the class II subfamily of tRNA synthases. Functioning as a monomer, AlaRS catalyzes the ATP-dependent attachment of alanine to a corresponding tRNA(Ala), thereby producing alanyl-tRNA(Ala). Defects in the gene encoding AlaRS may lead to an accumulation of misfolded proteins within the cell, ultimately leading to cell death. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of AlaRS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 701-968 mapping at the C-terminus of AlaRS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 701-968 mapping at the C-terminus of AlaRS of human origin |
| Entrez Gene | 16 |
|---|
Application
Western blot analysis of AlaRS expression in non-transfected 293T: (A), human AlaRS transfected 293T: (B), MCF7 (C), HeLa (D), K-562 (E) and Hep G2 (F) whole cell lysates.
Application
Western blot analysis of AlaRS expression in HeLa whole cell lysate.
Application
Western blot analysis of AlaRS expression in Caki-1 (A), K-562 (B) and Hep G2 (C) whole cell lysates.| Application Notes | recommended for detection of AlaRS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
