Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Amylase expression in mouse pancreas (A) and rat pancreas (B) tissue extracts.| Product Name | Amylase Antibody (H-281) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 212-492 of Amylase 2B of human origin |
| Modification Notes | The three types of Amylase found in human and mouse tissues are salivary, pancreatic and ovarian tumor. In humans there are two haplotypes consisting of very different numbers of salivary Amylase proteins. The short haplotype contains two pancreatic proteins, AMY2A and AMY2B and one salivary Amylase protein, AMY1C. The long haplotype consists of two salivary Amylase proteins, AMY1A and AMY1B. In mice, there are two apparently identical copies of AMY2A which specify pancreatic Amylase. The single copy of AMY1A is expressed in a tissue specific fashion in the salivary gland and the liver. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of precursor and mature Amylase 1 (salivary), 2A and 2B (pancreatic) of human origin, and Amylase 1 and 2a2-5 of mouse origin and the corresponding rat homolog WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 212-492 of Amylase 2B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 212-492 of Amylase 2B of human origin |
| Entrez Gene | 276 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Amylase expression in mouse pancreas (A) and rat pancreas (B) tissue extracts.| Application Notes | recommended for detection of precursor and mature Amylase 1 (salivary), 2A and 2B (pancreatic) of human origin, and Amylase 1 and 2a2-5 of mouse origin and the corresponding rat homolog WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
