Application
Western blot analysis of ROR1 expression in non-transfected (A) and human ROR1 transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic and membrane localization.| Product Name | ROR1 Antibody (P-288) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ROR1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The ROR-family receptor tyrosine kinases consist of two structurally related proteins, ROR1 and ROR2. These proteins are characterized by having intracellular tyrosine kinase domains, which are highly related to Trk-family kinases, extracellular Frizzled-like cysteine-rich domains (CRDs) and Kringle domains. The ROR family members are highly conserved among species, such as C. elegans, Drosophila, Xenopus and mammals. ROR1 and ROR2 are both involved in organogenesis with particular emphasis in neuronal differentiation. Increased expression of ROR1 in acute lymphoblastic leukemias (ALLs) as well as chronic lymphocytic leukemias (CLLs) implicate this protein as a potential tool for targeted immunotherapy in these diseases. ROR2 is involved in the Wnt-signalling pathway, and mutations in ROR2 lead to Brachydactyly type B and Robinow Syndrome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ROR1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of ROR1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 4919 |
|---|
Application
Western blot analysis of ROR1 expression in non-transfected (A) and human ROR1 transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic and membrane localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
