Application
Western blot analysis of PKC ν expression in non-transfected 293: (A), human PKC ν transfected 293: (B) and HeLa (C) whole cell lysates.| Product Name | PKC ν Antibody (Q-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PKC ν of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (å, ∫I, ∫II, ©, ∂, é, Ω, h, †, λ/î, µ and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC ∂ and é are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PKC &nu of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PKC ν of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 23683 |
|---|
Application
Western blot analysis of PKC ν expression in non-transfected 293: (A), human PKC ν transfected 293: (B) and HeLa (C) whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
