Application
Western blot analysis of C/EBP ζ expression in H460 whole cell lysate.| Product Name | C/EBP ζ Antibody (H-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of C/EBP ζ of human origin by WB, IP and ELISA |
| Modification Notes | CCAAT-enhancer binding proteins (C/EBP) are basic region/leucine zipper (bZIP) transcription factors selectively expressed during the differentiation of liver, adipose tissue, blood cells and pancreas. C/EBP family members include C/EBP å, C/EBP ∫, C/EBP ©, C/EBP ∂, C/EBP é and C/EBP Ω, all of which are capable of forming homodimers or heterodimers with each other. C/EBP Ω, also known as CBF2, NOC1, CHOP10 (C/EBP homologous protein 10), GADD153 (growth arrest and DNA damage-inducible gene 153) or HSP-CBF, is a ubiquitously expressed 1,054 amino acid protein that localizes to the nucleus and regulates transcription. More specifically, C/EBP Ω is believed to play a role regulating the expression of CRP (C-reactive protein). Unlike most C/EBP dimers, C/EBP Ω-containing dimers do not bind the classical C/EBP-binding consensus sequence. Rather, C/EBP Ω-containing dimers must associate with RBP-J˚ (another transcription factor) in order to accomplish binding to the C/EBP site. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of C/EBP &zeta of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of C/EBP ζ of human origin by WB, IP and ELISA |
| Entrez Gene | 10153 |
|---|
Application
Western blot analysis of C/EBP ζ expression in H460 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
