Application
Western blot analysis of HoxD9 expression in non-transfected 293T: (A), mouse HoxD9 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of HoxD9 expression in non-transfected: (A) and human HoxD9 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of HoxD9 expression in non-transfected control (A) and HoxD9 siRNA transfected (B) HeLa cells. Blot probed with HoxD9 (H-342): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of HoxD9 expression in HUV-EC-C (A) and FHs 173We (B) whole cell lysates.| Product Name | HoxD9 Antibody (H-342) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-342 representing full length HoxD9 of human origin |
| Modification Notes | The Hox proteins play a role in patterns of embryonic development and cellular differentiation by regulating downstream target genes. In vivo, the HoxD9 protein interacts with the autoregulatory and cross-regulatory enhancers of the murine HoxB1 and human HoxD9 genes. Specifically, the HoxD9 protein interacts with the human control region (HCR) of the HoxD9 gene, thus inducing transcription of the HoxD9 promoter. HoxD9 may be a multifunctional transcriptional regulator, as it contains different activation domains. Activation of HoxD9 depends on the structure of the target regulatory element, and results in differential cofactor interaction. The HoxD9 protein is expressed in the early stages of mouse joint development, primarily in the articular cartilage. HoxD9 transcripts are also detected in the synovial tissue of arthritic mice, but not in that of normal mice, suggesting that HoxD9 may have a role in the pathology of arthritis. Furthermore, the HoxD9 protein is highly expressed in the synoviocytes of patients with rheumatoid arthritis (RA), but not in osteoarthritis patients. The human HoxD9 protein is also differentially expressed in the human cervical cancer cell line HeLa, but is not expressed in the normal cervix and may thus play a role in tumorigenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of HoxD9 and, to a lesser extent, HoxA9, HoxB9 and HoxC9 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-342 representing full length HoxD9 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-342 representing full length HoxD9 of human origin |
| Entrez Gene | 3235 |
|---|
Application
Western blot analysis of HoxD9 expression in non-transfected 293T: (A), mouse HoxD9 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of HoxD9 expression in non-transfected: (A) and human HoxD9 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of HoxD9 expression in non-transfected control (A) and HoxD9 siRNA transfected (B) HeLa cells. Blot probed with HoxD9 (H-342): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of HoxD9 expression in HUV-EC-C (A) and FHs 173We (B) whole cell lysates.| Application Notes | recommended for detection of HoxD9 and, to a lesser extent, HoxA9, HoxB9 and HoxC9 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
