Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of CUL-1 expression in HeLa whole cell lysate.Application
Western blot analysis of CUL-1 expression in non-transfected 293T: (A), mouse CUL-1 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of CUL-1 expression in JAR (A) and HOS (B) whole cell lysates.Application
Western blot analysis of human recombinant CUL-1 fusion protein.| Product Name | CUL-1 Antibody (H-213) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 57-269 mapping near the N-terminus of cullin-1 (CUL-1) of human origin |
| Modification Notes | Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CUL-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 57-269 mapping near the N-terminus of cullin-1 (CUL-1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 57-269 mapping near the N-terminus of cullin-1 (CUL-1) of human origin |
| Entrez Gene | 8454 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of CUL-1 expression in HeLa whole cell lysate.
Application
Western blot analysis of CUL-1 expression in non-transfected 293T: (A), mouse CUL-1 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of CUL-1 expression in JAR (A) and HOS (B) whole cell lysates.
Application
Western blot analysis of human recombinant CUL-1 fusion protein.| Application Notes | recommended for detection of CUL-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
