Application
Western blot analysis of HRPAP20 expression in T-47D (A), Ramos (B), MDA-MB-231 (C), K-562 (D) and Raji (E) whole cell lysates.Application
Western blot analysis of HRPAP20 expression in T-47D (A) and MCF7 (B) whole cell lysates.| Product Name | HRPAP20 Antibody (FL-175) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-175 representing full length HRPAP20 of human origin |
| Modification Notes | HRPAP20 (UPF0240 protein C6orf66, hormone-regulated proliferation-associated protein 20) is a 175 amino acid protein encoded by the human gene C6ORF66. HRPAP20 is a novel hormone-regulated, proliferation-associated protein. In tumor cell lines, constitutive HRPAP20 expression enhanced proliferation and suppressed apoptosis, characteristics frequently associated with malignant progression. Invasive breast cancer cell lines and human breast tumor specimens express elevated HRPAP20, which can increase malignant cell invasion in transfection experiments using such cell lines as MCF-7 and MDA-MB-231. Transfection with HRPAP20 will increase secretion of matrix metalloproteinase-9 (MMP-9). Conversely, knockdown of HRPAP20 with small interfering RNA will reduce invasion and inhibit secretion of MMP-9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HRPAP20 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-175 representing full length HRPAP20 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-175 representing full length HRPAP20 of human origin |
| Entrez Gene | 29078 |
|---|
Application
Western blot analysis of HRPAP20 expression in T-47D (A), Ramos (B), MDA-MB-231 (C), K-562 (D) and Raji (E) whole cell lysates.
Application
Western blot analysis of HRPAP20 expression in T-47D (A) and MCF7 (B) whole cell lysates.| Application Notes | recommended for detection of HRPAP20 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
