Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of smooth muscle cells.Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing cytoplasmic localization.Application
Western blot analysis of Bax expression in RAW 264.7 whole cell lysate.| Product Name | Bax Antibody (Δ 21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-171 representing all but the C-terminal 21 amino acids of Baxα of mouse origin |
| Modification Notes | The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax (Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activity of Bcl-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Baxα and Baxβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-171 representing all but the C-terminal 21 amino acids of Bax&alpha of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-171 representing all but the C-terminal 21 amino acids of Baxα of mouse origin |
| Entrez Gene | 581 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic staining of smooth muscle cells.
Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing cytoplasmic localization.
Application
Western blot analysis of Bax expression in RAW 264.7 whole cell lysate.| Application Notes | recommended for detection of Baxα and Baxβ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
