Application
Western blot analysis of GCP3 expression in non-transfected: (A) and truncated human GCP3 transfected: (B) 293 whole cell lysates.| Product Name | GCP3 Antibody (H-303) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-303 mapping at the N-terminus of GCP3 of human origin |
| Modification Notes | The ©-Tubulin complex is composed of © Tubulin and the ©-Tubulin complex-associated proteins GCP2, GCP3, GCP4, GCP5 and GCP6, all of which are essential components of microtubule organizing centers. ©-Tubulin complex components are localized to both the centrosome, where they are involved in microtubule nucleation, and to the cytoplasm, where they exist as soluble complexes that can be recruited to the centrosome as needed. Although the GCP proteins are related, they have distinct roles which contribute to the proper function of the ©-Tubulin complex. GCP3 (©-Tubulin complex component 3), also known as TUBGCP3 or SPBC98, localizes to the centrosome and is a ubiquitously expressed 907 amino acid member of the ©-Tubulin complex. Like GCP2 and © Tubulin, GCP3 is conserved in all eukaryotes, suggesting that it is part of a core unit involved in eukaryotic microtubule nucleation. Three isoforms of GCP3 exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of γ-tubulin complex component 3 isoforms 1, 2, and 3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-303 mapping at the N-terminus of GCP3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-303 mapping at the N-terminus of GCP3 of human origin |
| Entrez Gene | 10426 |
|---|
Application
Western blot analysis of GCP3 expression in non-transfected: (A) and truncated human GCP3 transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of γ-tubulin complex component 3 isoforms 1, 2, and 3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
