Application
Western blot analysis of SGK3 expression in HeLa whole cell lysate.Application
Western blot analysis of SGK3 expression in A-375 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.| Product Name | SGK3 Antibody (X-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of SGK3 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Serine/threonine-protein kinase Sgk3 (SGK3), also designated serum/glucocorticoid regulated kinase 3, belongs to the Ser/Thr protein kinase family of proteins. The serum- and glucocorticoid-regulated kinase proteins are closely related to the Akt protein family. SGK1, a homolog of SGK3, activates ion channels, in particular potassium (K+) channels. SGK2 and SGK3 have been found to also be involved in this activation process, making all three of these proteins important regulators for cell proliferation, epithelial transport and neuromuscular excitability. SGK3 acts as a mediator of IL-3 dependent survival signals in the cell. It localizes to the early endosome and in vesicle-like structures. SGK3 is a widely expressed protein, but it is primarily detected in kidney, liver, pancreas, brain and heart. Phosphorylation of SGK3 at residue Ser 486 leads to an increase in SGK3 activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SGK3 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SGK3 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 23678 |
|---|
Application
Western blot analysis of SGK3 expression in HeLa whole cell lysate.
Application
Western blot analysis of SGK3 expression in A-375 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
