Application
Western blot analysis of VILIP-1 expression in mouse brain tissue extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Product Name | VILIP-1 Antibody (K-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of VILIP-1 of mouse and human origin by WB, IP, IF, IHC(P), ELISA |
| Modification Notes | The VSNL1 gene to the short arm of chromosome 2 encodes VILIP-1 (visinin-like protein 1). VILIP-1 contains four EF-hands and a double-stranded RNA-binding domain and is a member of the neuronal calcium sensor family, which is included in the calcium-binding protein superfamily. VILIP-1 is expressed in the membrane, cytoplasm and cytoskeleton of the sympathetic and parasympathetic neurons throughout the brain, except for the caudate-putamen region. The rate of VILIP-1 expression decreases significantly with age. VILIP-1 associates with actin in the cytoskeleton, which may translocate VILIP-1 to the membrane. VILIP-1 binds the 3’-untranslated region of trkB double-stranded mRNA in a calcium dependent manner. VILIP-1 associates with G protein-receptor kinase 1 and inhibits its binding to the membrane. VILIP-1 is involved in membrane calcium signaling and may play a role in the sensitivity of G-protein cascades to cytosolic. Decreased amounts of VILIP-1 were found in Alzheimer disease brains, suggesting it may play a role in the disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of VILIP-1 of mouse and human origin by WB IP IF IHC(P) ELISA |
|---|---|
| Tissue Specificity | recommended for detection of VILIP-1 of mouse and human origin by WB, IP, IF, IHC(P), ELISA |
| Entrez Gene | 7447 |
|---|
Application
Western blot analysis of VILIP-1 expression in mouse brain tissue extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
