Application
Western blot analysis of MCAT expression in 293T (A) and HeLa (B) whole cell lysates.| Product Name | MCAT Antibody (E-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of MCAT of human origin |
| Modification Notes | The biosynthesis of fatty acids can occur in the cytoplasm, via the type I fatty acid synthase complex, or in mitochondria, via the type II malonyl-CoA-dependent system. MCAT (mitochondrial malonyl CoA:ACP acyltransferase), also known as MT, MCT (mitochondrial malonyltransferase), fabD or FASN2C, is a member of the type II malonyltransferase family of proteins. Localizing to mitochondria, MCAT is encoded by a nuclear gene and, via an N-terminal localization signal, it is subsequently imported into mitochondria. MCAT functions in lipid metabolism and may be a component of a mitochondrial fatty acid synthase complex. More specifically, MCAT catalyzes the transfer of a malonyl group from malonyl-CoA to the mitochondrial acyl carrier protein (NDUFAB1), a subunit of respiratory complex 1. This reaction is essential in the initiation of the type II fatty acid biosynthesis system. Two isoforms of MCAT exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MCAT of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of MCAT of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of MCAT of human origin |
| Entrez Gene | 27349 |
|---|
Application
Western blot analysis of MCAT expression in 293T (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of MCAT of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
