Application
Western blot analysis of 15-LO2 expression in non-transfected: (A) and human 15-LO2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of 15-LO2 expression in non-transfected: (A) and human 15-LO2 transfected: (B) 293T whole cell lysates.| Product Name | 15-LO2 Antibody (H-55) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 146-200 mapping within an internal region of 15-LO2 of human origin |
| Modification Notes | Lipoxygenases are a family of enzymes which dioxygenate unsaturated fatty acids, thus initiating lipoperoxidation of membranes, the synthesis of signalling molecules as well as inducing structural and metabolic changes in the cell. The Lox enzymes in mammals, 12-LO and 15-LO, are classified with respect to their positional specificity of the deoxygenation of their most common substrate, arachidonic acid. The metabolism of arachidonic acid leads to the generation of biologically active metabolites that have been implicated in cell growth and proliferation, as well as survival and apoptosis. 15-Lipoxygenase acts in physiological membrane remodeling and the pathogenesis of atherosclerosis, inflammation and carcinogenesis. It is highly regulated and expressed in a tissue- and cell-type-specific fashion. IL-4 and IL-13 play important roles in transactivating the 15-LO gene. Overexpression of 15-LO type 1 in prostate cancer contributes to the cancer progression by regulating IGF-1R expression and activation. 15-lipoxygenase, type II (15-LO2) is important for the conversion of arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. It is a cytoplasmic protein expressed primarily in cornea, lung, hair and prostate. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of 15-lipoxygenase 2 splice variants a, b, c and d of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 146-200 mapping within an internal region of 15-LO2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 146-200 mapping within an internal region of 15-LO2 of human origin |
| Entrez Gene | 247 |
|---|
Application
Western blot analysis of 15-LO2 expression in non-transfected: (A) and human 15-LO2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of 15-LO2 expression in non-transfected: (A) and human 15-LO2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of 15-lipoxygenase 2 splice variants a, b, c and d of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
