Application
Western blot analysis of Emi2 expression in U-938-M whole cell lysate.| Product Name | Emi2 Antibody (H-62) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 482-543 mapping near the C-terminus of Emi2 of human origin |
| Modification Notes | F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein) type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. They are members of a larger family of proteins that are involved in the regulation of a wide variety of cellular processes (including the cell cycle, immune responses, signaling cascades and developmental events) through the targeting of proteins, such as cyclins, cyclin-dependent kinase inhibitors, I˚B-å and ∫-catenin, for proteasomal degradation. Emi2 (endogenous meiotic inhibitor 2), also known as FBXO43 (F-box only protein 43) or ERP1, is a 708 amino acid protein that contains one F-box domain and one IBR-type zinc finger. Playing an important role in protein modification, Emi2 is required for the establishment and maintenance of oocyte arrest at the second meiotic metaphase, an event that is crucial for fertilization. Specifically, Emi2 is thought to induce meiotic arrest by inhibiting the activity of the APC (anaphase-promoting complex), thereby preventing the progression of meiosis. Emi2 is subject to phosphorylation and ubiquitination, both of which promote its degradation by the proteasome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Emi2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 482-543 mapping near the C-terminus of Emi2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 482-543 mapping near the C-terminus of Emi2 of human origin |
| Entrez Gene | 286151 |
|---|
Application
Western blot analysis of Emi2 expression in U-938-M whole cell lysate.| Application Notes | recommended for detection of Emi2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
