Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of Factor B expression in human plasma whole cell lysate.Application
Western blot analysis of Factor B expression in non-transfected: (A) and human Factor B transfected: (B) 293 whole cell lysates.Application
Western blot analysis of Factor B expression in human truncated recombinant Factor B fusion protein.Application
Western blot analysis of Factor B expression in Human Factor B fusion protein whole cell lysate.| Product Name | Factor B Antibody (H-95) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 426-520 mapping within an internal region of Factor B of human origin |
| Modification Notes | The complement component proteins, C3, C4 and C5, are potent anaphylatoxins that are released during complement activation. Binding of these proteins to their respective G protein-coupled receptors, C3aR, C1R and C5aR, induces proinflammatory events, such as cellular degranulation, smooth muscle contraction, arachidonic acid metabolism, cytokine release, leukocyte activation and cellular chemotaxis. Complement Factor B, also designated Properdin Factor B or PBF2, is part of the alternate pathway of the complement system and is cleaved by Factor D into two fragments: Ba and Bb. Bb combines with complement Factor 3b to produce the C3 or C5 convertase and plays a role in the differentiation and proliferation of preactivated B lymphocytes, lysis of erythrocytes, stimulation of lymphocyte blastogenesis and rapid spreading of peripheral blood monocytes. Ba is important in inhibiting the proliferation of preactivated B lymphocytes. Adipsin, also designated complement Factor D, is a serine protease that cleaves complement Factor B and may be involved in obesity. Factor H controls the function of the alternative complement pathway. FHR-1 (complement Factor H related protein 1) may play a role in lipid metabolism. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Complement Factor B Bb Fragment of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 426-520 mapping within an internal region of Factor B of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 426-520 mapping within an internal region of Factor B of human origin |
| Entrez Gene | 629 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of Factor B expression in human plasma whole cell lysate.
Application
Western blot analysis of Factor B expression in non-transfected: (A) and human Factor B transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of Factor B expression in human truncated recombinant Factor B fusion protein.
Application
Western blot analysis of Factor B expression in Human Factor B fusion protein whole cell lysate.| Application Notes | recommended for detection of Complement Factor B Bb Fragment of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
