Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of Shh expression in non-transfected 293T: (A), human Shh transfected 293T: (B) and IMR-32 (C) whole cell lysates.Application
Western blot analysis of Shh expression in non-transfected: (A) and mouse Shh transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Ihh expression in non-transfected: (A) and human Ihh transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Ihh expression in non-transfected: (A) and mouse Ihh transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Shh expression in F9 whole cell lysate.Application
Western blot analysis of Dhh expression in non-transfected: (A) and human Dhh transfected: (B) 293T whole cell lysates.Application
Western blot analysis of recombinant N-terminal domain Shh.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Product Name | Shh Antibody (H-160) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 41-200 of Shh of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | The Drosophila segment polarity gene hedgehog (hh) encodes a precursor protein which undergoes autocleavage to generate amino and carboxy terminal peptides. Both proteins are secreted and appear to function in embryonic and imaginal disc patterning. Several vertebrate homologs of Drosophila hh have been identified. These include Sonic hedgehog (Shh) (alternatively designated Vhh-1), Desert hedgehog (Dhh) and Indian hedgehog (Ihh). Each contain amino terminal signal peptides and apparently function as secreted proteins involved in the mediation of various cell-cell interactions. Shh resembles Drosophila hh in that it is processed to generate an amino terminal secreted peptide that is retained at or near the cell surface and a carboxy terminal glycosylated more diffusible peptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | equine, canine, porcine and avian |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 41-200 of Shh of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 41-200 of Shh of human origin |
| Entrez Gene | 6469 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of Shh expression in non-transfected 293T: (A), human Shh transfected 293T: (B) and IMR-32 (C) whole cell lysates.
Application
Western blot analysis of Shh expression in non-transfected: (A) and mouse Shh transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Ihh expression in non-transfected: (A) and human Ihh transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Ihh expression in non-transfected: (A) and mouse Ihh transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Shh expression in F9 whole cell lysate.
Application
Western blot analysis of Dhh expression in non-transfected: (A) and human Dhh transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of recombinant N-terminal domain Shh.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.| Application Notes | WB:1:100~1:200 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
