Application
Western blot analysis of IgG expression in HL-60 (A) and MDA-MB-231 (B) whole cell lysates.| Product Name | IgG Antibody (H-270) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-290 mapping at the C-terminus of IgG3 of human origin |
| Modification Notes | IgG is a monomeric immunoglobulin. It is the most abundant immunoglobulin and is found in the blood and extracellular fluid. There are four subclasses of IgG: IgG-1, IgG-2, IgG-3 and IgG-4. IgG is composed of two heavy (©) chains and two light (˚ or ¬) chains. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Each IgG Fab fragment has two antigen binding sites. IgG molecules are involved in secondary immune response. They bind to several different kinds of pathogens, such as viruses, bacteria and fungi, and protect the body by complement activation (the classic pathway), opsonization for phagocytosis, and neutralization of toxins. In addition, IgG is the only isotype that can pass through the placenta, thereby providing protection to the fetus in the first weeks of life, before immune system of the fetus has developed. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of all IgG isotypes by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-290 mapping at the C-terminus of IgG3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-290 mapping at the C-terminus of IgG3 of human origin |
| Entrez Gene | 3502 |
|---|
Application
Western blot analysis of IgG expression in HL-60 (A) and MDA-MB-231 (B) whole cell lysates.| Application Notes | recommended for detection of all IgG isotypes by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
