Application
Western blot analysis of Orai1 expression in A-375 whole cell lysate.| Product Name | Orai1 Antibody (H-46) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 256-301 mapping at the C-terminus of Orai1 of human origin |
| Modification Notes | Orai1 (ORAI calcium release-activated calcium modulator 1), also known as ORAT1, CRACM1 (calcium release-activated calcium modulator 1) or TMEM142A (transmembrane protein 142A), is a 301 amino acid multi-pass membrane protein that belongs to the Orai family of proteins. Localizing to the plasma membrane, Orai1 plays an important role in store-operated calcium (SOC) entry, a process involving Ca2+ influx and replenishment of Ca2+ stores formerly emptied through the action of inositol 1,4,5-trisphosphate production and other Ca2+ mobilizing agents. Specifically, Orai1 functions as a pore subunit of the store-operated calcium release-activated calcium channel (CRAC) and is essential for proper function of the CRAC channel. CRAC channels are responsible for mediating calcium influx in T-cells and play an important role in the immune response. Mutations in the gene encoding Orai1 can result in severe combined immunodeficiency (SCID). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Orai1 of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react to a lesser extent w/Orai2 and Orai3 also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 256-301 mapping at the C-terminus of Orai1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 256-301 mapping at the C-terminus of Orai1 of human origin |
| Entrez Gene | 84876 |
|---|
Application
Western blot analysis of Orai1 expression in A-375 whole cell lysate.| Application Notes | recommended for detection of Orai1 of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react to a lesser extent w/Orai2 and Orai3 also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
